Can protein kinase R (PKR) regulate MeV IB morphology and NP phosphorylation

The measles virus has caused widespread illness and death since the early 1900s. Although an effective vaccine prevents most outbreaks, there is still no antiviral drug to treat active measles infections. One of the major host antiviral factors, protein kinase R (PKR), becomes activated upon viral infection and inhibits cellular protein synthesis. While this prevents viral replication, it also impairs cellular function. Using western blots, fluorescence microscopy, and DNA cloning, this project investigates a possible mechanism by which PKR can antagonize the measles virus by impeding the assembly of its replication factory, called inclusion bodies. Insights from this research could eventually guide the development of new therapies that strengthen the body’s immune defenses without harming cellular functions.